Cryptosporidium diagnosis in different groups of children and characterization of parasite species

Afiliação

(1)    Universidade Federal da Bahia, Programa de Pós-Graduação Stricto Sensu em Farmácia, Salvador, BA, Brasil.

(2)    Universidade Federal da Bahia, Hospital Professor Edgard Santos, Salvador, BA, Brasil.

(3)    Universidade Federal da Bahia, Departamento de Análises Clínicas e Toxicológicas, Salvador, BA, Brasil.

Resumo

Background: Microscopy and enzyme-linked immunosorbent assay (ELISA) are routinely used for Cryptosporidium diagnosis, without differentiating the parasite species. Methods: Children's feces were analyzed by modified Ziehl-Neelsen (mZN) and ELISA for Cryptosporidium diagnosis and by polymerase chain reaction-restriction fragment length polymorphism for species identification. Results: Cryptosporidium frequency was 2.6%. The sensitivity and specificity of ELISA were 85.7% and 99.7%, respectively, with excellent concordance with mZN (kappa=0.854). Parasite species were characterized as Cryptosporidium hominis (78.3%), Cryptosporidium felis (17.4%), and Cryptosporidium parvum (4.3%). Conclusions: Coproantigen ELISA is as efficient as mZN for Cryptosporidium diagnosis. Cryptosporidium genotyping suggests anthroponotic and zoonotic transmission to children.